Cyclic AMP-Binding Proteins: Inverse Relationship with Estrogen-Receptors in Hormone-Dependent Mammary Tumor Regression

Increased susceptibility of cytosol proteins to proteolytic digestion during regression of a hormone-dependent mammary tumor.

Regression of MTW9 mammary carcinoma, which consistently follows withdrawal of mammotropic hormones, was characterized by a rapid decrease of thymidine incorporation into DNA but only a slight reduction or uridine incorporation into RNA and amino acid incorporation into proteins. Within 24 hr of hormone withdrawal, cytosol proteins of MTW9 became more easily degraded by trypsin, alpha-chymotryp...

Comparison of estrogen receptors in hormone-dependent and hormone-independent Grunder strain mouse mammary tumors.

Hormone-dependent (HD) Grunder strain mouse mammary carcinomas contain a 65-kDa estrogen receptor (ER) with minor amounts of 50- and 35-kDa components which apparently still contain the intact hormone-binding (COOH-terminal) domain. When the HD tumors lose their hormonal dependence during serial transplantation, the hormone-independent (HI) transplants show an increase in 50- and 35-kDa compone...

Comparison of Estrogen Receptors in Hormone-dependent and Hormone- independent Grunder Strain Mouse Mammary Tumors1

Hormone-dependent (HD) Grunder strain mouse mammary carcino mas contain a 65-kDa estrogen receptor (ER) with minor amounts of 50and 35-kDa components which apparently still contain the intact hormone-binding (COOH-terminal) domain. When the HD tumors lose their hormonal dependence during serial transplantation, the hormoneindependent (HI) transplants show an increase in 50and 35-kDa com ponents...

Loss of nuclear cyclic AMP binding in cyclic AMP-unresponsive Walker 256 mammary carcinoma.

A marked increase of cyclic AMP-binding and protein kinase activities occurs in the nuclei of N6,02’-dibutyryl adenosine 3’:5’-monophosphate (Bt,cAMP)-responsive Walker 256 mammary carcinoma (W256) following incubation of the tumor slices with CAMP in uitro. The macromolecular fraction containing [“HIcAMP in the nuclei can be extracted with 1.0 M KC1 and identified by acrylamide gel electrophor...

Title 3 ' : 5 ' - Cyclic AMP Binding Proteins